Rp-hplc Method Development for the Quantification of Gabapentin in Formulations
نویسنده
چکیده
RP-HPLC METHOD DEVELOPMENT FOR THE QUANTIFICATION OF GABAPENTIN IN FORMULATIONS ABSTRACT A simple, precise and accurate RP-HPLC method was developed and validated for rapid assay of Gabapentin in tablet dosage form. Isocratic elution at a flow rate of 1 mL/min was employed on a symmetry Zodiac C18 (250mmx4.6mm,I.D., 5μm particle size) at ambient temperature. The mobile phase consisted of Methanol: Acetonitrile: Ortho phosphoric acid 65:33:2 % (V/V/V). The UV detection wavelength was 216nm and 20μl sample was injected. The retention time for Gabapentin was 3.7 2mins. The %RSD for precision and accuracy of the method was found to be less than 2%. The method was validated as per the ICH guidelines. The method was successfully applied for routine analysis of Gabapentin in tablet dosage form and bulk drug.
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